大蒜素对全脑缺血再灌注大鼠脑保护机制的研究

目的：通过大蒜素预处理，观察全脑缺血再灌注大鼠海马区ICAM-1 的表达，从而探讨大蒜素的脑保护机制。方法：雄性Wistar 大鼠30 只，随机分为5 组：假手术组、缺血再灌注组、缺血再灌注+ 大蒜素10、20、30 mg/kg 组。采用四血管闭塞法制备大鼠全脑缺血再灌注模型，于再灌注24 h 取出海马，硫堇染色观察海马组织的形态学改变，免疫组织化学染色测定海马CA1 区ICAM-1 免疫反应阳性细胞面积和积分光密度值。结果：通过给予大鼠全脑缺血8 min 再灌注24 h处理，海马CA1 区组织形态学改变显著，神经元密度明显降低；ICAM-1的表达显著增加。静脉给予大蒜素可使缺血再灌注海马组织形态学改变明显改善，存活神经元数目增加，ICAM-1 表达显著较少。结论：大蒜素可以通过减少ICAM-1 的表达抑制全脑缺血再灌注后的炎症损失从而发挥脑保护作用。

Cerebral Protection Mechanismof Allicin on Cerebral Ischemia Reperfusionin Rats

Objective:Through the observation of allicin preconditioning, observing the expression on cerebral ischemia-reperfusionin ICAM-1 area of hippocampus in rats, to study the mechanismof cerebral protection of allicin.Methods:30 male Wistar rats, wererandomly divided into 5 groups: sham operation group, ischemia reperfusion group, ischemia reperfusion+garlicin 10, 20, 30 mg/kggroup. By four vessel occlusion methods for the preparation of rats with global cerebral ischemia reperfusion model in 24 h after reperfusion,removing the hippocampus, thionine staining to observe morphological changes of hippocampal tissue, Immunohistochemical stainingwas measured hippocampal CA1 area of ICAM-1 immunoreactivity and integral optical density of positive cells.Results:By givingcomplete cerebral ischemia 8min reperfusion in rats 24h, morphology of hippocampal CA1 area organization changed significantly, neurondensity decreased significantly; the expression of ICAM-1 increased significantly. Intravenous administration of allicin can make ischemiaand morphology of hippocampal tissue perfusion changes significantly improved, the number of survival neurons increased, theexpression of ICAM-1 was significantly less.Conclusion:Allicin can reduce ICAM-1 by inhibiting the expression and play a role in brainprotection after cerebral ischemia - reperfusion inflammation loss.