LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication |
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Authors: | Rui Pedro Gal?o Ashwin Chari Isabel Alves-Rodrigues Daniela Lob?o Antonio Mas Christian Kambach Utz Fischer Juana Díez |
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Institution: | 1.Department of Experimental and Health Sciences, Universitat Pompeu Fabra, 08003 Barcelona, Spain;2.Department of Biochemistry, Biocenter, University of Wuerzburg, Am Hubland, D-97074 Wuerzburg, Germany;3.Centro Regional de Investigaciones Biomédicas—Facultad de Medicina, Universidad de Castilla La Mancha, 02006 Albacete, Spain;4.Biomolecular Research, Paul Scherrer Institut, CH5232 Villigen, Switzerland |
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Abstract: | LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3′-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions. |
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Keywords: | BMV LSm1-7 RNA virus Sm proteins translation replication |
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