The in vitro uptake of tritiated nucleic acid precursors by Babesia spp. of cattle and mice |
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Authors: | A D Irvin E R Young R E Purnell |
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Institution: | ARC Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, U.K. |
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Abstract: | Irvin A. D., Young E. R. and Purnell R. E. 1978. The in vitro uptake of tritiated nucleic acid precursors by Babesia spp. of cattle and mice. International Journal for Parasitology8: 19–24. Blood and mice infected with Babesia microti and B. rodhaini, and from cattle infected with B. divergens and B. major, was incubated in Eagles medium for 24 h in the presence of tritiated purines and pyrimidines. Uptake of these compounds was assessed by liquid scintillation counting and by autoradiography. Hypoxanthine, adenosine and adenine were readily incorporated by all four species of parasites. Thymine, thymidine and uridine were generally not incorporated. Uptake of 3H]hypoxanthine by B. microti occurred within minutes of exposure to the precursor. The amount of 3H]hypoxanthine incorporated by B. rodhaini-infected erythrocytes was proportional to the percentage of parasitized cellsThe results suggest that structural analogues of hypoxanthine and other purines may be incorporated and act against intra-erythrocytic Babesia. |
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Keywords: | tritium thymine thymidine uridine adenine adenosine hypoxanthine purine pyrimidine nucleic acid autoradiography liquid scintillation counting mice cattle erythrocyte |
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