Temperature dependence of ESR spectra of spin-labelled met-hemoglobin. |
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| Authors: | Vesna Nöthig-Laslo JN Herak S Maričić |
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| Institution: | Institute “Rudjer Bo?kovi?”, Zagreb, Croatia, Yugoslavia;Institute of Immunology, Zagreb, Croatia, Yugoslavia |
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| Abstract: | The maleimide spin-label, firmly fixed to the protein, was used to study conformation changes of various met-hemoglobin derivatives. The temperature dependence of the rotational correlation time shows a distinct conformation change in aquomet-hemoglobin at about 25 degrees C. The other met-hemoglobin derivatives studied (fluoro-, cyano-, aquomet-complexed with inositol hexaphosphate) and carbonmonoxy-hemoglobin exhibit no conformation changes in the temperature range from 0-50 degrees C. |
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