Characterization of photosynthetically active duckweed (Wolffia australiana) in vitro culture by Respiration Activity Monitoring System (RAMOS) |
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Authors: | Henrik Rechmann Andrea Friedrich Dara Forouzan Stefan Barth Heide Schnabl Manfred Biselli Robert Boehm |
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Affiliation: | Division Juelich, Universtity of Applied Science Aachen, Ginsterweg 1, Juelich, Germany. |
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Abstract: | The feasibility of oxygen transfer rate (OTR) measurement to non-destructively monitor plant propagation and vitality of photosynthetically active plant in vitro culture of duckweed (Wolffia australiana, Lemnaceae) was tested using Respiration Activity Monitoring System (RAMOS). As a result, OTR proofed to be a sensitive indicator for plant vitality. The culture characterization under day/night light conditions, however, revealed a complex interaction between oxygen production and consumption, rendering OTR measurement an unsuitable tool to track plant propagation. However, RAMOS was found to be a useful tool in preliminary studies for process development of photosynthetically active plant in vitro cultures. |
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Keywords: | Duckweed Plant in vitro culture Process development Respiration Activity Monitoring System Wolffia australiana |
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