Nineteen novel microsatellites in Chinese lake gudgeon Sarcocheilichthys sinensis Bleeker, 1971 |
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| Authors: | Y.‐B. Shen R.‐Q. Wang J.‐Q. Ren H.‐T. Fu X.‐M. Yang J. Li |
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| Affiliation: | 1. Jiangsu Postdoctoral Program, Suzhou Shenghang Eco‐technology Development Co., Ltd., Suzhou, China;2. Aquaculture Post‐doctoral Mobile Stations, Wuxi Fishery College, Nanjing Agricultural University, Wuxi, China;3. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China |
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| Abstract: | Nineteen polymorphic microsatellites isolated from a partial genomic DNA library of Sarcocheilichthys sinensis enriched for CA repeats were developed. Variability was tested on 48 unrelated individuals collected from Jiangsu province, China. Observed heterozygosity ranged from 0.200 (Ss236) to 1.00 (Ss223, Ss224), whereas the expected heterozygosity ranged from 0.213 (Ss232) to 0.939 (Ss224). Shannon's information index ranged from 0.405 (Ss236) to 2.988 (Ss224). Sixteen of the 19 microsatellites conformed to Hardy–Weinberg equilibrium. These microsatellites can be used for the evaluation of genetic parameters in populations to supply valuable information for conservation management. |
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