禽流感H5N1病毒感染BALB/c小鼠后多器官病变初探

为了研究禽流感H5N1病毒在各个器官的增殖和病理变化,在生物安全实验室,我们将禽流感H5N1病毒通过尾静脉接种BALB/C小鼠。结果小鼠在不经过适应的情况下,直接感染发病,甚至死亡。在观察的7天内,感染小鼠临床症状主要表现呼吸急促,体温、体重下降。尸检表现肺出血,心外膜坏死以及肝脏的坏死。组织病理检查表现心、肝、肺等多器官的病变。肺的病变伴有纤维化的弥漫性肺泡损伤;心肌外膜大量淋巴细胞浸润、坏死;肝细胞大量坏死,淋巴细胞浸润。心、肝的坏死病变在H5N1禽流感病毒相关的研究中未见报道。经过对各个组织器官的病毒载量的检测,未发现病毒在各个病变组织中的复制。免疫组化的检测,各个组织中也未检出阳性的细胞反应。因此,我们认为H5N1禽流感病毒感染小鼠引起多个器官组织的损伤,甚至死亡,不是病毒在器官的复制,而可能是病毒感染小鼠,产生炎症细胞因子的高度表达,损伤多个器官组织所致。

Study of the multiple-organ pathology for BALB/c Mice Infectedwith Avian Influenza A (H5N1) virus

To study the pathology of H5N1 avian influenza virus, H5N1 avian influenza virus was inoculated the tail intravenous to the BALB/c male mice and the mice were infected and died. With 1-3 days, the mice began to show acute respiratory distress, decreased activity, less food and water intake, weight and temperature declined. The death of the mice was occured in 2-3days. From 4 to 7 days, the clinical symptom of the mice was resumed. The infected mice showed lung hemorrhage, epicardium and liver necrosis. In histopathology, the main lesion was consolidated pulmonary tissue. The lumina of alveoli and bronchioles were variably filled with protein-rich edema, fluid, fibrin, erythrocytes and cell debris. Extrarespiratory lesions also were found in the mice. There were accumulation of lymphocytes in the epicardium and the liver. Up to the present the epicardium lesion and the hepatic lesion have not been reported in the H5N1 infected mice. The virus titers were not detected in the heart, liver, spleen, kidney and brain tissues by inoculated into embryonated hens' eggs. The virus antigens were not detected by immunohistochemistry in the 6 organ tissues. Our results suggested that the many organ tissues necrosis in the H5N1 virus infected mice might be caused by more cytokine responses, as result multiple-organ dysfunction syndrome, not caused by the virus replication in the organ tissues.