Quantitative evaluation of the lengths of homobifunctional protein cross-linking reagents used as molecular rulers |
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| Authors: | Nora S. Green Emil Reisler K.N. Houk |
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| Affiliation: | Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA |
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| Abstract: | Homobifunctional chemical cross-linking reagents are important tools for functional and structural characterization of proteins. Accurate measures of the lengths of these molecules currently are not available, despite their widespread use. Stochastic dynamics calculations now provide quantitative measures of the lengths, and length dispersions, of 32 widely used molecular rulers. Significant differences from published data have been found. |
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| Keywords: | Protein cross-linking cross-linking reagents molecular rulers stochastic dynamics |
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