Protease inhibitors from Streptomyces violascens

Summary Production of protease inhibitors from Streptomyces violascens U 10 600 was studied in quiescent flasks, shake flasks, and fermentors. A starting pH in the medium between pH 6 and 7 was the most favourable for the process. Both trypsin and chymotrypsin inhibitors were produced. Maximum inhibitor activity was attained after 3 days of cultivation in a protein powder medium at 18°C with pH control to pH 5 during the latter part of the process.