Genetic loci associated with field resistance to late blight in offspring of Solanum phureja and S.tuberosum grown under short-day conditions |
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Authors: | M Ghislain B Trognitz Ma del R Herrera J Solis G Casallo C Vásquez O Hurtado R Castillo L Portal M Orrillo |
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Institution: | (1) Department of Crop Improvement and Genetic Resources, Centro International de la Papa (CIP), PO Box 1558, Lima 12, Perú e-mail: m.ghislain@cgiar.org Tel.: +51-1-349-6017, Fax: +51-1-317-5326, PE |
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Abstract: | Field resistance to late blight – a fungal disease caused by Phytophthora infestans – has been genetically characterized by analyzing trait-marker association in a Solanum phureja (phu)×dihaploid Solanum tuberosum (dih-tbr) population. Trait data were developed at three locations over a 3-year period under natural infection pressure. RAPD (random
amplified polymorphic DNA) and AFLP (amplified fragment length polymorphism)markers were used to develop anonymous genetic
linkage groups subsequently anchored to potato chromosomes using mapped RFLP (restriction fragment length polymorphism), SSR
(single sequence repeats) and AFLP markers. RFLP and SSR markers achieved the most-accurate anchoring. Two genetic maps were
obtained, with 987.4 cM for phu and 773.7 cM for dih-tbr. Trait-marker association was revealed by single-marker and interval mapping analyses. Two important QTLs (quantitative trait
loci) were detected on chromosomes VII and XII as a contribution from both parents, totalling up to 16% and 43%, respectively,
of the phenotypic variation (PH). One additional QTL was detected on chromosome XI (up to 11% of the PH) as a contribution
from the phu parent, and three others were detected on chromosome III (up to 13% of the PH), chromosome V (up to 11% of the PH) and chromosome
VIII (up to 11% of the PH) as a contribution from the dih-tbr parent. Our results reveal new genetic loci of the potato genome that contribute to resistance to late blight. We postulate
that some of these loci could be related to plant growth under short-day conditions.
Received: 5 July 2000 / Accepted: 17 November 2000 |
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Keywords: | Phytophthora infestans Potato Genetic mapping Quantitative trait loci Disease resistance |
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