A partially purified β-glucosidase from Bifidobacterium adolescentis converts cycasin to a mutagenic compound |
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Authors: | Y.J. Choi C.J. Kim G.E. Ji |
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Affiliation: | Departments of Food Science and Nutrition, Sook-Myung Women's University, Seoul;Hallym University, Chun-Chon, Korea |
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Abstract: | Y.J. CHOI, C.J. KIM AND G.E. JI. 1996. β-Glucosidase was extracted from sonicated Bifidobacterium adolescentis Int-57 and partially purified by Sepharose CL-6B gel-filtration and DEAE-cellulose ion-exchange chromatography. The partially purified enzyme was confirmed to convert cycasin to a mutagen in the Ames and SOS chromotests. β-Glucosidase negative strains were unable to activate cycasin mutagenically. |
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