An alcohol dehydrogenase ribozyme |
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Authors: | Tsukiji Shinya Pattnaik Swetansu B Suga Hiroaki |
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Institution: | Department of Chemistry, University at Buffalo, State University of New York, Buffalo, New York 14260-3000, USA. |
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Abstract: | We report an RNA molecule that exhibits activity analogous to that of alcohol dehydrogenase (ADH). Directed in vitro evolution was used to enrich nicotinamide adenine dinucleotide (NAD+)-dependent redox-active RNAs from a combinatorial pool. The most active ribozyme in the population forms a compact pseudoknotted structure and oxidizes an alcohol seven orders of magnitude faster than the estimated spontaneous rate. Moreover, this ADH RNA was coupled with a redox relay between NADH and flavin adenine dinucleotide to give a NAD+-regeneration system. Our demonstration of the redox ability of RNA adds support to an RNA-based metabolic system in ancient life. |
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