| Changes in Thermostability of Photosystem Ⅱ and Leaf Lipid Composition of Rice Mutant with Deficiency of Light-harvesting Chlorophyll a/b Protein Complexes |
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| 引用本文： | Yunlai Tang Mei Chen Yinong Xu Tingyun Kuang. Changes in Thermostability of Photosystem Ⅱ and Leaf Lipid Composition of Rice Mutant with Deficiency of Light-harvesting Chlorophyll a/b Protein Complexes[J]. 植物学报(英文版), 2007, 49(4): 515-522. DOI: 10.1111/j.1744-7909.2007.00433.x |
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| 作者姓名： | Yunlai Tang Mei Chen Yinong Xu Tingyun Kuang |
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| 作者单位： | [1]College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621000, China [2]Photosynthesis Research Center, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China |
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| 基金项目： | Supported by the Frontier Project of the Knowledge Innovation Engineering of the Chinese Academy of Sciences （KSCXZ-SW-326）,Acknowledgement We are grateful to Professor Zhang Jingliu in the Institute of Plant Physiology ＆ Ecology, Shanghai Institutes of Biological Sciences, the Chinese Academy of Sciences, for donating the seeds of rice. |
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| 摘 要： | We studied the difference in thermostability of photosystem Ⅱ （PSII） and leaf lipid composition between a T-DNA insertion mutant rice （Oryza sativa L.） VG28 and its wild type Zhonghuau. Native green gel and SDS-PAGE electrophoreses revealed that the mutant VG28 lacked all light-harvesting chlorophyll a/b protein complexes. Both the mutant and wild type were sensitive to high temperatures, and the maximal efficiency of PSII photochemistry （FJ Fm） and oxygen-evolving activity of PSII in leaves significantly decreased with increasing temperature. However, the PSII activity of the mutant was markedly more sensitive to high temperatures than that of the wild type. Lipid composition analysis showed that the mutant had less phosphatidylglycerol and sulfoquinovosyl diacylglycerol compared with the wild type. Fatty acid analysis revealed that the mutant had an obvious decrease in the content of 16：1t and a marked increase in the content of 18：3 compared with the wild type. The effects of lipid composition and unsaturation of membrane lipids on the thermostability of PSII are discussed.
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| 关 键 词： | 水稻 光系统Ⅱ 捕光叶绿素a/b蛋白复合体缺乏型突变 叶片 类脂成分 热稳定性 高温 |
| 修稿时间： | 2006-03-302006-05-18 |
Changes in Thermostability of Photosystem II and Leaf Lipid Composition of Rice Mutant with Deficiency of Light-harvesting Chlorophyll a/b Protein Complexes |
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| Yunlai Tang ,Mei Chen,Yinong Xu, Tingyun Kuang. Changes in Thermostability of Photosystem II and Leaf Lipid Composition of Rice Mutant with Deficiency of Light-harvesting Chlorophyll a/b Protein Complexes[J]. Journal of integrative plant biology, 2007, 49(4): 515-522. DOI: 10.1111/j.1744-7909.2007.00433.x |
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| Authors: | Yunlai Tang Mei Chen Yinong Xu Tingyun Kuang |
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| Affiliation: | College of Life Science and Engineering, Southwest University of Science and Technology;, Mianyang 621000, China;Photosynthesis Research Center, Institute of Botany, the Chinese Academy of Sciences;, Beijing 100093, China |
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| Abstract: | We studied the difference in thermostability of photosystem II (PSII) and leaf lipid composition between a T‐DNA insertion mutant rice (Oryza sativa L.) VG28 and its wild type Zhonghua 11. Native green gel and SDS‐PAGE electro‐phoreses revealed that the mutant VG28 lacked all light‐harvesting chlorophyll a/b protein complexes. Both the mutant and wild type were sensitive to high temperatures, and the maximal efficiency of PSII photochemistry (Fv/ Fm) and oxygen‐evolving activity of PSII in leaves significantly decreased with increasing temperature. However, the PSII activity of the mutant was markedly more sensitive to high temperatures than that of the wild type. Lipid composition analysis showed that the mutant had less phosphatidylglycerol and sulfoquinovosyl diacylglycerol compared with the wild type. Fatty acid analysis revealed that the mutant had an obvious decrease in the content of 16:1t and a marked increase in the content of 18:3 compared with the wild type. The effects of lipid composition and unsaturation of membrane lipids on the thermostability of PSII are discussed. |
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| Keywords: | high temperature lipid composition light-harvesting a/b protein complexes photosystem II rice. |
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