Expression of active alpha-N-acetylglucosaminidase/TAT Chimerae in cultured Spodoptera frugiperda cells |
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| Authors: | Bandsmer Judith C Campbell Tessa N Cheyne Ian Choy Francis Y M |
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| Affiliation: | Southern Alberta Cancer Research Institute, Dept. of Oncology, University of Calgary, Calgary, AB, Canada. |
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| Abstract: | We examined the production and secretion of fusion constructs containing alpha-N-acetylglucosaminidase, the enzyme deficient in Sanfilippo B, and either wildtype TAT or modified TAT in cultured Spodoptera frugiperda cells. All constructs exhibited successful expression of active enzyme, suggesting the future possibility of utilizing TAT/alpha-N-acetylglucosaminidase chimerae in enzyme replacement therapy. |
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