A spectrophotometric method for the assay of threonine dehydratase |
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| Authors: | L Davis |
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| Affiliation: | 1. Zijin School of Geology and Mining, Fuzhou University, Fuzhou 350108, China;2. College of Civil Engineering, Fuzhou University, Fuzhou 350108, China;3. Fujian-Taiwan Science and Technology Cooperation Base of Fujian Province on Intelligent Geoenvironmental Engineering, Fuzhou 350116, China;4. School of Civil Engineering, Wuhan University, Wuhan 430072, China;5. School of Civil Engineering and Architecture, Henan University, Kaifeng 475004, China;1. Department of Agriculture, Sri Guru Teg Bahadur Khalsa College, Sri Anandpur Sahib, India;2. Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, India;3. DAV University, Jalandhar, India |
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| Abstract: | The enzymic conversion of β-hydroxy α-amino acids to α-keto acids by ThrDH (sheep liver) was followed by a direct spectrophotometric method at 230 mμ. The assay was more sensitive to changes in keto acid concentrations than the 2,4-dinitrophenylhydrazone method because of the absence of the high and variable blanks of the latter method. However, in the applicable range of concentrations both methods gave the same results. This procedure is not handicapped by the physical properties inherent in the coupled reactions. |
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