Plasmid profile and construction of a small shuttle vector in <Emphasis Type="Italic">Laribacter hongkongensis</Emphasis> |
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| Authors: | Patrick C Y Woo Shirley S L Ma Jade L L Teng Maria W S Li Susanna K P Lau Kwok-yung Yuen |
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| Institution: | State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong. |
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| Abstract: | Among 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies. |
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| Keywords: | Laribacter hongkongensis Plasmid profile Sequence Shuttle vector Transformation |
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