Co-expression of bacterial hemoglobin overrides high glucose-induced repression of foreign protein expression in Escherichia coli W3110 |
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Authors: | Yeon Kyu Kim Dong Gyun Kang Suk-Soon Choi Ji Hyeon Kim Jae Chun Chung Hyung Joon Cha |
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Affiliation: | Department of Chemical Engineering & Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea. |
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Abstract: | Co-expression of Vitreoscilla hemoglobin (VHb) can enhance production of foreign proteins in several microorganisms, including Escherichia coli. Production of foreign proteins [green fluorescent protein (GFP) and organophosphorous hydrolase (OPH)] has been examined in two typical industrial E. coli strains, W3110 (a K12 derivative) and BL21 (a B derivative). In particular, we investigated the effects of VHb co-expression and media glucose concentration on target protein production. We employed the nar O(2)-dependent promoter for self-tuning of VHb expression based on the natural changes in dissolved O(2) levels over the duration of culture. Foreign protein production in strain BL21 was decreased by a high glucose concentration but co-expression of VHb had no effect on this. In contrast, co-expression of VHb in strain W3110 overrode the glucose-induced repression and resulted in steady expression of foreign proteins. |
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Keywords: | catabolite repression green fluorescent protein hemoglobin organophosphorous hydrolase |
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