Intrinsic properties of so-called dormant probiotic bacteria, determined by flow cytometric viability assays |
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| Authors: | Lahtinen Sampo J Ouwehand Arthur C Reinikainen Johanna P Korpela Jaakko M Sandholm Jouko Salminen Seppo J |
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| Institution: | Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland. sajola@utu.fi |
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| Abstract: | Plate counting and four culture-independent flow cytometric assays were used to determine the viability and intrinsic properties of three probiotic strains during storage. The strains showed reduction in plate counts but were able to maintain esterase activity, intact cytoplasmic membrane, and pH gradient. The apparently uncultivable probiotic cells were active and stress resistant. |
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