| SRFA法构建水稻DNA指纹图谱 |
| |
| 引用本文： | 陈洪 王振山. SRFA法构建水稻DNA指纹图谱[J]. 生物工程学报, 1996, 12(3): 266-269 |
| |
| 作者姓名： | 陈洪 王振山 |
| |
| 作者单位： | 中国科学院遗传研究所，北京农业大学农学系，中国科学院遗传研究所 北京 100101 中国科学院动物研究所生殖生物学国家重点实验室 北京 100080，北京 100101 |
| |
| 基金项目： | “863”计划经费支持 |
| |
| 摘 要： | 水稻基因组DNA用PstⅠ酶切同时与人工接头连接后,使用选择性引物进行PCR扩增,琼脂糖凝胶电泳检测所构建的水稻DNA指纹图谱。结果表明在JX17和ZYQ8间以及5种野生稻间均存在DNA多态性片段。
|
| 关 键 词： | SRFA 水稻 DNA指纹图谱 |
Construction of Rice DNA Fingerprinting by SRFA |
| |
| Chen Hong Wang Zhenshan Zhu Lihuang. Construction of Rice DNA Fingerprinting by SRFA[J]. Chinese journal of biotechnology, 1996, 12(3): 266-269 |
| |
| Authors: | Chen Hong Wang Zhenshan Zhu Lihuang |
| |
| Abstract: | After rice genome DNA was restricted with Pst I and ligated with adaptor meanwhile. DNA fingerprinting was constructed by selective primers for PCR amplification. The DNA fingerprinting was detected through agarose gel electrophoresis. This result shows that there are DNA polymorphic fragments between ZYQ8 and JX17, also in five wide rice accession (Oryza rufipogon) one another. |
| |
| Keywords: | SRFA rice DNA fingerprinting |
| 本文献已被 CNKI 维普 等数据库收录！ |
