从反胶束溶液中反萃牛血清白蛋白的研究

由近红外光谱证实,十六烷基三甲基溴化铵(CTAB)/正己醇-正辛烷反胶束溶液是牛血清白蛋白(BSA)增溶于非极性有机溶剂的较理想中介。研究了反萃液酸度、离子强度和种类等参数对BSA反萃率的影响,过低的溶液酸度导致BSA变性。适宜的反萃液为:1.0～2.0mol/L KBr,pH4.3～4.9。蛋白质的紫外光谱表明,BSA分子在料液,反胶束溶液和反萃液中的构象大致相同。此外,探讨了反胶束溶液循环使用的效率问题。最后,通过采用适当的相比,成功地实现了蛋白质的回收和浓缩。

Study on Bovine Serum Albumin Recovery from Reversed Micellar Solutions

From near-infrared spectra, it was confirmed that CTAB/hexanol-octane reversed micellar solution is a suitable medium for bovine serum albumin (BSA) solubiliza-tion. The effects of various factors, such as pH, the type and concentration of salt in stripping solution, on BSA back extraction were studied. The suitable conditions for stripping have been ascertained,i, e. , 1. 0-2. 0mol/L KBr,pH = 4. 3-4. 9. Spectroscopic studies showed that no significant change is observed in the structure of the main chain of BSA after extraction and back extraction. In addition, the problem how long the reversed micellar solution can be recycled have been examined. Finally, the recovery and concentration of protein were successfully obtained with suitable phase ratio.