固定化细胞分布对动力学影响的模型化研究

通过对固定化Gluconobacter oxydans和Bacillus cereus的活细胞系统的研究,提出了固定化细胞不均匀分布模型,将此类分布与均匀分布(最劣分布模型)进行比较,分析了它们对固定化细胞内部的基质浓度分布、有效速率因子和选择率的影响,指出不均匀分布和均匀分布对于该固定化活细胞系统的动力学行为无显著影响,这一模型分析结果在实验中得以验证。通过无因次化分析,建立了适用于固定化生物催化剂动力学研究的模型方法。

Studies on Modelizing the Effects of Immobilized Cell Distribution on Kinetics

A nonuniform distribution model of immobilized cells was established by the studies on immobilized Gluconobacter oxydans and Bacillus cereus living cells. It was compared with uniform distribution model. Effects of the two typical cell distribution on substrate distribution in immobilized biocatalyst, effective rate factor and selective yield were discussed. No obvious difference was observed between the effects of uniform cell distribution and that of nonuniform cell distribution on kinetics of immobilized G. oxydans and B. cereus living cells, which was then confirmed in experiments. The conclusion is also fit for the studies on kinetics of other immobilized biocatalyst system.