葡萄糖的添加对昆虫细胞Sf21悬浮生长的影响

添加葡萄糖对昆虫细胞Sf21(Spodoptera frugiperda)悬浮生长的影响，发现补加糖量在lg／L时·能明显提高细胞生长的速度和最高密度，细胞最高密度由2．5×10⁴／ml增加到4.9×10⁶／ml; 朴加糖量在2g／L时，则有显著的抑制作用，即使增加接种密度．细胞生长的最高密度也只有2．1×10⁶／ml。当采取流加葡萄糖方法来培养细胞时，则其生长的最大密度可提高到5．2×10⁶／ml。

The Effect of Glucose Addition on the Suspension Growth of Insect Cell Sf21

It was studied the effect of glucose addition on the suspension growth of insect cell Sf21. Compared with no glucose addition, it was found that at the addition of Ig/L, the growth rate of Sf21 was enhanced, the maximal density was increased from 2. 5 x 106/ml to 4- 9x106/ml; at the addition of 2g/L, the inhibition effect appeared, even the inoculum density was increased, the maximal density of Sf21 cell was only 2.1 x 106/ml. When the glucose-fed batch culture was used, the maximal density of Sf21 culture could be more increased to 5. 2x106/ml. In the compare of glucose and lactate metabolism under these conditions, it was found that at the addition of 2g/L, the metabolism of glucose and lactate was different from other conditions. However, it was difficult to explain the inhibition effect of the glucose at high concentration.