检测香蕉花叶心腐病病原CMV的PAS—ELISA方法的改进

作为一种快速、敏感的免疫学方法,ELISA已被广泛地用于植物病毒的研究与应用性检测方面。在实验室中用PAS-ELISA检测香蕉花叶心腐病病原CMV已被证明是一种获得无CMV组培苗的有效手段。在香蕉无病毒组培苗工厂化大批量生产中,我们应用此法作为质量控制的手段之一,剔除病苗,保证产品不带CMV,获得一定成效。但同时我们也发现,香蕉组织中含有引起非特异性吸附反应的物

The Improvement of PAS-ELISA for Detection of CMV-free Germplasm in Larger Scale Micropropagation of Banana Plants

Owing to. the application of a routine ELISA test for detection of CMV-free germplasm in larger scale production of the micropropagated banana plants,we found severe false positive interference which caused high rate of germplasm loss. A modified test was developed by addition of sulphite in the solution for preparation of antigen. Statistical data showed that serious interference occured in the samples of the banana sucker and the first 1 - 2 generation of the micropropagated plantlet by using routine technique, and the false positive reaction were almost completely eliminated with the modified method. The dilution curve of purified CMV and TMV verified that there is no difference between the routine and modified methods in term of the sensitivity.