高山红景天愈伤颗粒组织悬浮培养动力学及工艺的研究

研究了高山红景天愈伤组织颗粒悬浮培养过程的生长及红景天甙合成的规律，发现红景天甙合成与细胞生长偶联，两者之间的关系表示为q=5．38×10^-3μ。其产率系数Yx/s及维持系数m分别为0．66g·g^-1和0．033g·(g·d)^-1。在3.5L气升式反应器中研究了培养过程的操作特性，发现“发泡”现象几乎不存在，氧传递系数K1a的变化不同常规植物细胞的悬浮培养．愈伤组织颗粒在培养过程中经历一个由小变大，后叉变小的过程。

Studies on Kinetics and Technology of Rhodiola sachalinensis A.Bor Callus Suspension Culture

The kinetics of growth and salidroside synthesis for Rhodiola schalinensis A. Bor callus suspension culture were investigated,which resulted in a maximum biomass of 14. 02 g - L-1 with a salidroside content of 0. 566%. The synthesis of salidroside was found to be coupled with cell growth. Moreover, cell yield coefficient and maintenance coefficient were estimated as 0. 66g - g-1 and 0. 033g - (g - d)-1 respectively. Callus suspension culture was also conducted in a 3. 5L air-lift bioreactor and the technological characteristics were investigated as well. It was found that the viscosity of the medium did not increase greatly during the culture like the conventional plant cell culture. The sizes of callus grains experienced a change from small to greater then back to smaller over the culture process. In addition, the oxygen transfer coefficient (KLa) of the reactor during the culture was also discussed.