4-N-Alkanoyl and 4-N-alkyl gemcitabine analogues with NOTA chelators for 68-gallium labelling |
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Authors: | Jesse Pulido Maria de Cabrera Adam J. Sobczak Alejandro Amor-Coarasa Anthony J. McGoron Stanislaw F. Wnuk |
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Affiliation: | 1. Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, United States;2. Department of Biomedical Engineering, Florida International University, Miami, FL 33199, United States |
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Abstract: | The conjugation of 4-N-(3-aminopropanyl)-2′-deoxy-2′,2′-difluorocytidine with 2-(p-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (SCN-Bn-NOTA) ligand in 0.1?M Na2CO3 buffer (pH 11) at ambient temperature provided 4-N-alkylgemcitabine-NOTA chelator. Incubation of latter with excess of gallium(III) chloride (GaCl3) (0.6?N AcONa/H2O, pH?=?9.3) over 15?min gave gallium 4-N-alkylgemcitabine-NOTA complex which was characterized by HRMS. Analogous [68Ga]-complexation of 4-N-alkylgemcitabine-NOTA conjugate proceeded with high labeling efficiency (94%–96%) with the radioligand almost exclusively found in the aqueous layer (~95%). The high polarity of the gallium 4-N-alkylgemctiabine-NOTA complex resulted in rapid renal clearance of the 68Ga-labelled radioligand in BALB/c mice. |
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Keywords: | Nucleosides Gemcitabine Prodrugs NOTA PET imaging 68-Gallium |
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