Assay of glucoprotein formation from uridine diphosphate glucose by a rapid filter-paper technique. |
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Authors: | C R Krisman R Barengo |
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Affiliation: | 1. Instituto de Investigaciones Bioquimicas “Fundacion Campomar”, Obligado 2490, Buenos Aires (28), Argentina;2. Facultad de Ciencias Exactas y Naturales, Obligado 2490, Buenos Aires (28), Argentina |
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Abstract: | A rapid, simple, sensitive and reproducible filter-paper assay was developed to follow the glucose transfer from uridine diphosphate [14C]glucose to protein or protein-bound oligosaccharides. This method could be useful for following the formation of any glycoprotein.Rectangles of trichloroacetic acid-treated filter paper are introduced into a test tube containing the reaction mixture. The papers are submitted to a batch washing-procedure that ensures the removal of the compounds that are not trichloroacetic acid-precipitable. After scintillation counting, the paper can be reused to study the acid lability of the glucoprotein. |
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