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Biocatalytic preparation of chiral epichlorohydrins using recombinant<Emphasis Type="Italic">Pichia pastoris</Emphasis> expressing epoxide hydrolase of<Emphasis Type="Italic">Rhodotorula glutinis</Emphasis> |
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| Authors: | Hee Sook Kim Jae-Hwa Lee Sunghoon Park Eun Yeol Lee |
| Institution: | (1) Department of Food Science and Technology, Kyungsung University, 608-736 Busan, Korea;(2) Department of Bioscience and Biotechnology, Silla University, 617-736 Busan, Korea;(3) Department of Chemical and Biochemical Engineering, Pusan National University, 609-735 Busan, Korea |
| Abstract: | The use of enantioselective hydrolysis for preparing chiral epichlorohydrins was investigated using recombinantPichia pastoris with the enantioselective epoxide hydrolase ofRhodotorula glutinis. The rate of the recombinant epoxide hydrolase-catalyzed enantioselective hydrolysis of racemic epichlorohydrins was enhanced by the addition of 5% (v/v) Tween 20. Enantiopure (R)-epichlorohydrins with an enantiopurity of 100%ee and a yield of 26% were obtained within 5 min from 50 mM racemates. |
| Keywords: | enantioselective hydrolysis epichlorohydrin epoxide hydrolase Rhodotorula glutinis Pichia pastoris |
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