| 外源性线粒体DNA在细胞内的重建（简报） |
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| 引用本文： | 刘芳,张兰,等.外源性线粒体DNA在细胞内的重建（简报）[J].实验生物学报,2002,35(3):243-247. |
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| 作者姓名： | 刘芳 张兰 |
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| 作者单位： | [1]北京大学第三医院 [2]首都医科大学宣武医院药理研究室，北京脑老化重点实验室，北京100053 |
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| 摘 要： |
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| 关 键 词： | 外源性线粒体DNA p^0细胞 转线粒体DNA细胞 mtDNA 细胞功能 细胞融合 |
Repopulation with exogenous mitochondria in the cell] |
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| Fang Liu,Lan Zhang,Lin Li.Repopulation with exogenous mitochondria in the cell][J].Acta Biologiae Experimentalis Sinica,2002,35(3):243-247. |
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| Authors: | Fang Liu Lan Zhang Lin Li |
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| Institution: | Department of Pharmacology, Xuan-Wu Hospital of Capital University of Medical Sciences, Beijing Key Laboratory for Brain Aging, Beijing 100053. |
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| Abstract: | To create human mitochondrial DNA (mtDNA)-transferred cells as cell model of mitochondria defects-related diseases, platelet of normal young and old subjects were isolated as donor of mtDNA, then fused with mtDNA-depleted cells (rho0 cells) under induction of PEG1500. Auxtrophy test, cytochrome c oxidase activity assay and PCR amplification of mtDNA were done to confirm the transferring of mtDNA. Cell clones were visible in the medium 10 to 15 days after fusion, which grew well in medium lacking uridine, had positive COX activity and contained objective fragment of mtDNA by PCR, opposite to rho0 cells. Transferring of mtDNA to rho0 cells was identified, and mtDNA-transferred cell models were successfully created. |
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