Insights into maize genome editing via CRISPR/Cas9 |
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| Authors: | Astha Agarwal Pranjal Yadava Krishan Kumar Ishwar Singh Tanushri Kaul Arunava Pattanayak Pawan Kumar Agrawal |
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| Affiliation: | 1.Indian Council of Agricultural Research- Indian Institute of Maize Research,New Delhi,India;2.Department of Biology,Stanford University,Stanford,USA;3.International Centre for Genetic Engineering and Biotechnology,New Delhi,India;4.Indian Council of Agricultural Research- Vivekananda Parvatiya Krishi Anusandhan Sansthan,Almora,India;5.National Agricultural Science Fund, Indian Council of Agricultural Research,New Delhi,India |
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| Abstract: | Maize is an important crop for billions of people as food, feed, and industrial raw material. It is a prime driver of the global agricultural economy as well as the livelihoods of millions of farmers. Genetic interventions, such as breeding, hybridization and transgenesis have led to increased productivity of this crop in the last 100 years. The technique of genome editing is the latest advancement in genetics. Genome editing can be used for targeted deletions, additions, and corrections in the genome, all aimed at genetic enhancement of crops. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated protein 9 (CRISPR/Cas9) system is a recent genome editing technique that is considered simple, precise, robust and the most revolutionary. This review summarizes the current state of the art and predicts future directions in the use of the CRISPR/Cas9 tool in maize crop improvement. |
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