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Effect of Ammonia on Cellular pH of Anabaena cylindrica Determined with 31P NMR Spectroscopy
Authors:Ohmori  Masayuki; Oh-hama  Tamiko; Furihata  Kazuo; Miyachi  Shigetoh
Institution:1Ocean Research Institute, University of Tokyo Minamidai 1-15-1, Nakano-ku, Tokyo 164, Japan
2Institute of Applied Microbiology, University of Tokyo Yayoi 1-1-1, Bunkyo-ku, Tokyo 113, Japan
Abstract:The pH changes in the blue-green alga (cyanobacterium) Anabaenacylindrica caused by addition of ammonia were investigated using31P NMR spectroscopy. A pH shift of 0.9 or more was observedwhen 30 nM NH4OH was added to the cell suspension, but no significantcellular pH change was observed with 50 mM NH4CI, a concentrationhigh enough to stimulate dark CO2 fixation of this alga. Thechange in cellular pH does not seem to cause ammonia-inducedstimulation of dark CO2 fixation. (Received June 22, 1985; Accepted January 10, 1986)
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