Arginine and ornithine decarboxylases in embryogenic and non-embryogenic carrot cell suspensions |
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Authors: | Natalia Loukanina Trevor A. Thorpe |
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Affiliation: | (1) Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada, T2N 1N4 |
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Abstract: | The in vivo activities of arginine and ornithine decarboxylases, key enzymes in the biosynthesis of putrescine and thus polyamines, were measured in three different cell lines of carrot (Daucus carota) during growth and somatic embryogenesis. The activities of these two enzymes differed in the different cell lines in the presence of various levels of auxin (2,4 dichlorophenoxy acetic acid), but was highest during periods of active cell division. During somatic embryo development, the activities of both enzymes were highest during globular stage formation. Thus, both enzymes were found to be active during growth and somatic embryogenesis and could contribute to polyamine biosynthesis. |
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Keywords: | Daucus carota Cell culture growth Polyamine biosynthesis Putrescine formation Somatic embryogenesis |
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