Preparation of specific antisera to estradiol 17-glucuronide |
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| Authors: | T Nambara M Numazawa T Tanaka T Ohkubo |
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| Affiliation: | 1. Key Laboratory of Science and Engineering for Marine Ecological Environment, the First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China;2. Laboratory of Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China |
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| Abstract: | The preparation and antigenic properties of three different types of estradiol 17-glucuronide-bovine serum albumin (BSA) conjugates in which the haptens are linked to the carrier protein through the C-2 (or C-4) position have been described. All antibodies raised against these antigens in rabbits possessed high affinity (KA = 2.22 × 109–2.58 × 109M−1) and excellent specificity to estradiol 17-glu-curonide, exhibiting little cross-reactivities for other estrogen glucuronides and sulphates (<1%) and no cross-reactions for free estrogens and other steroids (< 0.001%) with an only exception of testosterone 17-glucuronide (0.86–11.5%). |
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