He-T family DNA sequences in the Y chromosome of Drosophila melanogaster share homology with the X-linked Stellate genes |
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Authors: | Olga N Danilevskaya Elena V Kurenova Maria N Pavlova Dmitrii V Bebehov Andrew J Link Akihiko Koga Ann Vellek Daniel L Hartl |
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Institution: | (1) Department of Genetics, Washington University School of Medicine, 63110-1095 St. Louis, MO, USA;(2) Institute of Molecular Genetics, USSR Academy of Science, 123182 Moscow, USSR;(3) Department of Genetics, Harvard Medical School, 02115 Boston, MA, USA |
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Abstract: | The genome of Drosophila melanogaster contains a class of repetitive DNA sequences called the He-T family, which is unusual in being confined to telomeric and heterochromatic regions. The specific He-T fragment designated Dm665 was cloned in yeast by selection for an autonomously replicating sequence (ARS). Dm665 contains a restriction fragment length polymorphism (RFLP) that is specific to males and thus derives from the Y chromosome. Deletion mapping using X-Y translocations indicates that sequences homologous to Dm665 occur in at least one major cluster in each arm of the Y chromosome. Among 20 yeast artificial chromosome (YAC) clones containing Drosophila sequences homologous with Dm665, four clones derive from defined regions of the long arm of the Y and two from the short arm. The sequence of Dm665 is 2443 bp long, consists of 59% A+T, and contains no significant open reading frames or direct or inverted repeats. However, Dm665 contains a region of 650 bp that shares homology with portions of the X-linked locus Stellate.by W. Hennig |
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