Pilot-scale multi-stage multi-feeding continuous ethanol fermentation using non-sterile cane molasses

Summary Non-aseptic fermentation of a 28 brix cane molasses solution was successfully carried out in a pilot-scale 5-stage multi-feeding continuous system for 30 days. The effluent ethanol concentration, overall volumetric productivity and sugar conversion yield averaged 8.54 % (v/v), 5.35 g/L-hr and 92.4 % of theoretical, respectively.