Immunogold localization of a developmentally regulated,tapetal-specific, 15 kDa lily anther protein |
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| Authors: | R A Balsamo J -L Wang K J Eckard C -S Wang E M Lord |
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| Institution: | (1) Department of Botany and Plant Sciences, University of California, 92521 Riverside, CA, USA;(2) Present address: Department of Surgery, UCLA Medical Center, Los Angeles, California, USA;(3) Present address: Agricultural Biotechnology Laboratories, National Chung-Hsing University, Taichung, Taiwan |
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| Abstract: | Summary We have confirmed that the LLA-15 polypeptide ofLilium longiflorum is (a) tapetum specific with some expression possible in the adjacent middle layer cells and (b) relatively abundant as evidenced by the high density of gold particles localized to the tapetal cells. We have established that the protein is cytoplasmic and not associated with organelles, membranes, extracellular matrix or wall. We also report an amino acid composition of the molecule and a partial sequence which bears no resemblance to any protein yet described.Abbreviations BSA
bovine serum albumin
- PMSF
phenyl methyl sulfonyl fluoride |
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| Keywords: | Lily Tapetum Anther Protein Immunogold localization |
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