Micropropagation and plant regeneration from embryogenic callus of Miscanthus sinensis |
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Authors: | Qi Xiang Zhang Yu Sun Heng Kang Hu Bei Chen Chun Tao Hong Hai Peng Guo Yin Hui Pan Bing Song Zheng |
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Affiliation: | (1) School of Forestry and Biotechnology, Zhejiang A & F University, Linan, Hangzhou, 311300, People’s Republic of China;(2) College of Landscape Architecture and Art, Jiangxi Agricultural University, Nanchang, 330045, China;(3) Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Zhejiang, 311300, China;(4) Forestry Bureau of Longyou County, Longyou, 324400, Zhejiang, China;(5) School of Forestry and Biotechnology, Dong Hu Campus, Zhejiang A & F University, 88 North Circle Road, Linan, 311300, People’s Republic of China; |
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Abstract: | Miscanthus sinensis (Poaceae) is a typical perennial giant grass of East Asia. Due to its high photosynthetic efficiency, low input requirements, and high biomass production, M. sinensis shows outstanding potential as a biofuel feedstock. However, the lack of an efficient tissue culture system may limit its utilization potential. Different explants of M. sinensis were evaluated to develop an efficient tissue culture system. Shoot apices from in vitro-germinated seedling explants were tested for adventitious bud proliferation. The highest level of proliferation (multiplication coefficient 6.69) was obtained when shoot apices were cultured on Murashige and Skoog (MS) medium supplemented with 1.0 mg L−1 6-benzyladenine (BA), 2.0 mg L−1 kinetin, 0.05 mg L−1 α-naphthalene acetic acid (NAA), 3% sucrose, and 0.8% agar. The highest rooting percentage (95.4%) was obtained when adventitious buds were cultured on half-strength MS medium supplemented with 0.2 mg L−1 NAA, 3% sucrose, and 0.8% agar. Significant differences were found in the formation of embryogenic callus among different explant types. The embryogenic callus derived from epicotyls had the highest regeneration capacity when cultured on a medium supplemented with 2.0 mg L−1 2,4-dichlorophenoxyacetic acid, 0.5 mg L−1 BA, and 0.1 mg L−1 thiamine. Under these conditions, the callus induction percentage was 82%. |
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