Isolation of viable egg cells of perennial ryegrass (Lolium perenne L.) |
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Authors: | H M van der Maas M A C M Zaal E R de Jong F A Krens J L Van Went |
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Institution: | (1) Department of Cell Biology, Centre for Plant Breeding and Reproduction Research, CPRO-DLO, P.O. Box 16, NL-6700 AA Wageningen, The Netherlands;(2) Department of Plant Cytology and Morphology, Wageningen Agricultural University, Wageningen |
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Abstract: | Summary Isolation of viable egg cells of perennial ryegrass (Lolium perenne L.) has been accomplished. After an enzyme incubation, ovules disintegrated into loose cells upon mechanical manipulation. The egg cells could be identified between the bulk of sporophytic cells derived from the macerated ovules. The morphology of the isolated egg cell corresponds to the morphology of the egg cell in situ and is comparable to the morphology of egg cells of other monocotyledons and angiosperms. Two hours after isolation the egg cells were still viable. The protocol proved reproducible and the yield was determined at 10%. |
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Keywords: | Egg cell in situ Egg cell isolation Embryo sac Perennial ryegrass |
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