酯酶试验与聚合酶链反应鉴别中间普氏菌的比较研究

目的 研究脂酶试验和常规生化方法与聚合酶链反应比较在鉴别中间普氏菌Pi中的敏感度和特异度。方法 对207株牙周临床分离的产黑色素G厌氧杆菌分别进行脂酶试验、常规生化法鉴定和16srRNA特异引物PCR鉴定。结果 207株实验菌中PCR鉴定出Pi97株，脂酶试验检测出Pi126株，其中有85株PCR阳性，其敏感度为87．6％，特异度为63．7％。常规生化方法和脂酶试验共同鉴定出Pi80株，其中有55株PCR为阳性，其敏感度为67％，特异度为86．4％。结论 脂酶与常规生化方法对Pi的鉴别能力低于PCR（ P＜0．05），尚不能作为Pi菌种的可靠鉴定方法。

COMPARISON OF LIPASE TEST AND PCR FOR IDENTIFICATION OF PREVOTELLA INTERMEDIA

Objective:This study is coparison of the lipase test and PCR for identification of Prevotella intermedia by investigating the sensitivity and specificity Methods:207 strains of black pigmented Gram negtive anaerobic rods isolated from clinical periodontal pockes were detected.Bacterial identification was performed by lipase test,conventional biochemical methods and PCR using specific primers with sequences of 16s rRNA.Results:Among 207 strains,97 stains were identified by PCR as P.intermedia and 126 strains were identified by lipase test as P intermedia However, only 85 of 126 strains were also identified by PCR as P.intermedia.The sensitivity was 87 6% and the specificity was 62 7%.Combination of conventional biochemical methods and lipase test identified 80 strains as P.intermedia,only 65 of 80 strains were identified by PCR as P.intermedia,its sensitivity was 67% and the specificity was 86 4%.Conclusions:The efficacy of lipase test and conventional biochemical methods was lower than that of PCR (P<0 05).Lipase test and conventional biochemical methods can't be used as reliable identification methods.