池蝶蚌和三角帆蚌谷胱甘肽S转移酶基因表达特征

采用RT-PCR方法克隆获得池蝶蚌(Hyriopsis schlegerlii)和三角帆蚌(H.cumingii)GST片段,两种蚌的GST核苷酸序列相同,推导其编码92个氨基酸;经与不同物种进行氨基酸序列比对,显示其与软体动物、两栖动物和高等哺乳动物的GST具有高度同源性。RT-PCR半定量分析表明,GST在两种蚌的肝、闭壳肌等11种组织中除肠以外均有表达,肝中表达量较大;注射嗜水气单胞菌(Aeromonas hydrophila)后,三角帆蚌血细胞中GST表达量先降低后升高,而池蝶蚌血细胞中GST表达量先升高后降低,且在感染3h、6h、9h和24h时表达量约为三角帆蚌的2倍。

Expression of Glutathione S-Transferase Gene in Hyriopsis schlegerlii and H. cumingii

In this study,we used RT-PCR to clone glutathione s-transferase (GST) fragments from Hyriopsis schlegerlii and H. cumingii. The two species had the same sequence which encoded a peptide of 92 amino acids. They shared high GST homology with mollusks,amphibian and mammals when comparing different spices at a polypeptide level. GST was detected in all tissues except for intestine,especially higher expression of GST in liver was observed. Result showed that the GST expression in haemocyte was declined at the first stage and then increased in H. cumingii,while its expression pattern was opposite in H. schlegerlii and the expression level of GST in the latter was approximately two times of that in H. cumingii at 3 h,6 h,9 h and 24 h after infection by Aeromonas hydrophila.