Cytochrome P-450 isoforms in the liver of rats treated with phenobarbital, 3-methylcholanthrene and Aroclor 1254 studied by monoclonal antibodies

Seven types of monoclonal antibodies to cytochrome P-450 were obtained from the rat liver. Liver microsomal samples from intact rats and those pretreated with phenobarbital, 3-methylcholanthrene, Aroclor 1254, pregnenalone carbonitrile, beta-naphthoflavone and imidazole were stained with these antibodies using immunoblotting technique. The study made it possible to draw the following conclusions. Firstly, two types of these antibodies react with two cytochrome P-450 isoforms, P-450b and P-450 PB/PCN-E. Secondly, two types of antibodies react with three cytochrome P-450 isoforms: P-450a, P-450b and P-450PB/PCN-E. Antibodies of the latter three clones react with two cytochrome P-450 isoforms: P-450c and P-450d. Antibodies of all seven clones can be used for immunomorphological identification of cytochrome P-450 on rat liver paraffin sections.