Metabolism of juvenile hormone in cultures of ovaries and fat body in the cockroachperiplaneta Americana |
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Authors: | Sams Gary R. Cocchiaro Gerald F. Bell William J. |
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Affiliation: | (1) Department of Physiology and Cell Biology, University of Kansas, 66045 Lawrence, Kansas;(2) Department of Entomology, University of Kansas, 66045 Lawrence, Kansas;(3) Present address: Department of Physiological Chemistry, University of Wisconsin, 53706 Madison, Wisconsin |
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Abstract: | Summary The time course of juvenile hormone (JH) metabolism is examined in cultures ofPeriplaneta americana fat body and ovaries in medium containingManduca sexta carrier protein or cockroach hemolymph. In the absence ofM. sexta carrier protein or cockroach hemolymph, both tissues extensively catabolize exogenous [3H]JH in the medium. Addition of the carrier protein or hemolymph to the culture system prevents the hydrolysis of the hormone in the medium. Within the tissues JH is degraded whether or not carrier protein or hemolymph is present which suggests that the protective role of these molecules is exclusively extracellular. Incubation of [3H]JH with medium preconditioned with tissue results in destruction of the hormone. This suggests that the fat body secretes esterases into the medium. In contrast, the ovarioles hydrolyze the hormone by means of cell-associated enzyme. The relationship of these phenomena to insect development is discussed. This work supported by NSF Grant PCM 76-02229 and University of Kansas Biomedical Sciences Grant RR-07037. |
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Keywords: | juvenile hormone metabolism fat body ovary carrier protein Periplaneta americana hemolymph |
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