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Immunofluorescence location using monoclonal antibodies of prolactin receptors in mammary epithelial cells of lactating rabbits
Authors:T Seddiki  J Djiane  M Ollivier-Bousquet
Affiliation:Laboratoire de Biologie cellulaire et moléculaire, I.N.R.A.-C.R.J., Jouy-en-Josas.
Abstract:Immunofluorescent staining of PRL receptors on frozen sections of lactating rabbit mammary gland with a monoclonal antibody (IgG1 M110) shows that receptors are localized in the cytoplasm of epithelial cells and on short portions of plasma membrane. In vivo treatment by bromocriptine or in vitro treatment of mammary tissue slices by monensin modifies localization of receptors.
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