|
|||||
|
|
| 四倍体大燕麦×六倍体裸燕麦的杂种F1的产生及鉴定 | |
| 引用本文： | 赵云云,周小梅,杨才.四倍体大燕麦×六倍体裸燕麦的杂种F1的产生及鉴定[J].植物学通报,2003,20(3):302-306. |
| 作者姓名： | 赵云云 周小梅 杨才 |
| 作者单位： | 1. 首都师范大学生物系,北京,100037 2. 山西大学生命科学与技术学院,太原,030006 3. 河北省张家口坝下农科所,张家口,075131 |
| 基金项目： | 河北省自然科学基金资助项目 |
| 摘 要： | 本研究以四倍体大燕麦 (AvenamagnaL .)做母本 ,六倍体裸燕麦 (AvenanudaL .)做父本进行杂交 ,利用幼胚拯救技术获得了杂种F1,并对其后代形态特征进行了观察 ;对杂种F1同工酶图谱和DNA指纹图谱进行了分析。杂种F1形态特征偏亲本或介于双亲之间 ;同工酶研究表明多数F1具有双亲互补酶带 ;RAPD分析不同引物扩增产物F1呈共显性或偏父、偏母。这些结果表明F1为真杂种 |
| 关 键 词： | 四倍体大燕麦 六倍体裸燕麦 远缘杂交 同工酶 RAPD标记 |
| 修稿时间： | 2002年9月18日 |
Production of Hybrid F1 Between Avena magna and Avena nuda and It's Identification |
|
| Abstract: | The intergeneric hybrid between Avena magna and Avena nuda was successfully obtained by means of embryoes rescue. The hybrids F 1 were intermediate between the parents in morphology, and their traits resembled either parents at different developmental stages. The zymogram pattern of hybrids F 1 mainly displayed complementary pattern of the two parents. Genomic DNAs was used for RAPD analysis from parents and hybrids. Some primers show co_dominant or maternal_like or the some as male parent. The results indicated that the hybrid F 1 we obtained is true hybrid. |
| Keywords: | Avena magna Avena nuda Distant hybridization Isozymes analysis RAPD |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |