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A mitochondrial protein fraction catalyzing transport of the K+ analog T1+.
Authors:J J Diwan  R Paliwal  E Kaftan  R Bawa
Affiliation:Biology Department, Rensselaer Polytechnic Institute, Troy, NY 12180-3590.
Abstract:A protein fraction has been obtained from detergent-solubilized mitochondrial membranes by its affinity for quinine, an inhibitor of K+ transport. A peptide derived from the predominant 53 kDa protein in this fraction is found to be identical in sequence to a portion of aldehyde dehydrogenase. Antigenically unrelated bands at 97, 77, 57, and 31 kDa are also seen on polyacrylamide gels. Observations utilizing a fluorescent probe entrapped in the lumen of membrane vesicles indicate that the reconstituted protein fraction imparts permeability to the K+ analog Tl+. These and other findings suggest that the affinity purified fraction includes a cation transport catalyst.
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