Tissue- and stage-specific expression of a soybean (Glycine max L.) seed-maturation,biotinylated protein |
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Authors: | Hsing Yue-ie C Tsou Chih-hua Hsu Tze-fu Chen Zuey-ying Hsieh Kim-leong Hsieh Jaw-shu Chow Teh-yuan |
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Institution: | (1) Institute of Botany, Academia Sinica, Taipei, T, aiwan;(2) Department of Agronomy, National Taiwan University, Taipei, Taiwan |
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Abstract: | A cDNA clone GmPM4 which encodes mRNA species in mature or dry soybean seeds was characterized. DNA sequence analysis shows that the deduced polypeptides have a molecular mass of 68 kDa. GmPM4 proteins have a relatively high amino acid sequence homology with a major biotinylated protein isolated from pea seeds, SBP65, but both of these proteins differ markedly from that of presently known biotin enzymes. The accumulation of GmPM4 mRNA is detectable in the leaf primodium and the vascular tissues of the hypocotyl-radicle axis of mature seeds, and the GmPM4 proteins are present at high levels in dry and mature soybean seeds, but not in fresh immature seeds. It degrades rapidly at the early stage of seed germination. These proteins are boiling-soluble and biotinylated when they are present endogenously in soybean seeds; however, the same recombinant protein expressed in Escherichia coli is boiling-soluble, but it is not biotinylated. |
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Keywords: | developmental expression localization recombinant protein seed maturation |
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