An improved micropropagation system for Chrysanthemum based on Pluronic F-68-supplemented media |
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Authors: | M Khehra K C Lowe M R Davey J B Power |
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Institution: | (1) Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, University Park, NG7 2RD Nottingham, United Kingdom |
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Abstract: | Supplementation of MS-based medium containing 4.4 M 6-benzyladenine and 2.7 M -naphthaleneacetic acid with 0.001–0.1% (w/v) of the non-ionic, co-polymer surfactant, Pluronic F-68, significantly (p<0.05) increased the mean fresh weight gain of cultured leaf explants of chrysanthemum (Dendranthema grandiflora) Tone Maid and Early Charm by a maximum of 74% and 34%, respectively. The percentage of individual explants giving adventive shoots was also stimulated by Pluronic F-68; for cv. Early Charm, 0.001% (w/v) Pluronic F-68 induced the maximum response, whereas for cv. Tone Maid, the maximum response occurred with 0.01% surfactant. Shoot regeneration from explants was also enhanced at these concentrations of surfactant, compared to explants cultured in the absence of Pluronic F-68.Abbreviations BA
6-benzyladenine
- MS
Murashige & Skoog 1962
- NAA
-naphthaleneacetic acid |
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Keywords: | Dendranthema grandiflora syn Chrysanthemum morifolium surfactant |
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