DNA methylation in different origin clonal offspring from a mature <Emphasis Type="Italic">Sequoiadendron giganteum</Emphasis> genotype |
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Authors: | Olivier Monteuuis Sylvie Doulbeau Jean-Luc Verdeil |
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Institution: | (1) CIRAD-BIOS, UPR 39, Campus international de Baillarguet TA 10/C, Baillarguet, 34398 Montpellier Cedex 5, France;(2) IRD, UMR DIAPC, 911 Avenue Agropolis BP 6450, 34394 Montpellier Cedex 5, France;(3) CIRAD-BIOS, UMR DAP PHIV, TA 40/02 Avenue Agropolis, 34398 Montpelier Cedex 5, France |
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Abstract: | A meristem-issued rejuvenated line was obtained in 1986 from a 100-year-old Sequoiadendron giganteum tree and has been since then micropropagated in tissue culture conditions maintaining its juvenile-like characteristics.
By contrast, grafts and rooted microcuttings from the same genotype planted in outdoor conditions for several years exhibited
mature foliage traits and the grafts started to produce cones, which are obvious indicators of physiological aging. These
three different clonal lines were compared with regard to global DNA methylation assessed by HPLC. The in vitro rejuvenated
line showed a much higher level of DNA methylation (23% as average value) than the two other outdoor origins from the same
clone which displayed similar degrees of global methylation (average values of 13.4% for the grafts and 13.8% for the cuttings).
Overall these DNA global methylation values obtained for the first time in S. giganteum are consistent with the level of methylation reported for many plants using the same HPLC protocols. The fact that shoots
exhibiting a juvenile-like leaf morphology can be characterized by higher DNA methylation than mature-like ones is discussed
in relation to physiological aging, referring to other studies on the same topic. |
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Keywords: | Aging Coniferous species DNA methylation Maturational traits Phase change |
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