Synthesis of sterigmatocystin on a chemically defined medium by species ofAspergillus andChaetomium |
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| Authors: | S. E. Barnes T. P. Dola Dr J. W. Bennett D. Bhatnagar |
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| Affiliation: | (1) Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana, USA;(2) Southern Regional Research Center, New Orleans, Louisiana, USA;(3) Department of Biology, Tulane University, 2000 Percival Stern Hall, 70118 New Orleans, LA, USA |
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| Abstract: | Sterigmatocystin (ST) is a secondary metabolite and a principal mycotoxin known to be produced by over 30 species of filamentous fungi. It is also one of the late intermediates in aflatoxin biosynthesis. We have tested the ability of 7 species ofAspergillus, including 4 strains ofA. versicolor, one species ofBipolaris, and two species ofChaetomium, to produce ST on a sucrose-salts-phenylalanine defined medium as well as on three complex substrates. Highest ST production in our survey was by a strain ofA. versicolor grown on wheat, whereas, the highest ST production on defined medium was byC. cellulolyticum. To our knowledge, this is the first report of ST production byC. cellulolyticum on any substrate. In precursor feeding studies, resting cultures of wild typeA. nidulans andA. versicolor were unable to biotransform O-methylsterigmatocystin (OMST), the last known intermediate in aflatoxin biosynthesis. These results suggest that ST is the end product of polyketide metabolism in the strains tested. |
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| Keywords: | Aflatoxin biosynthesis Aspergillus Chaetomium Defined medium Sterigmatocystins |
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