The displacement effect of phenylalanine on lysozyme elution in hydrophobic interaction chromatography |
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| Authors: | Klimchak RJ Wang S |
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| Institution: | (1) Department of Chemical and Biochemical Engineering, Reuters University, New Brunswick, New Jersey 08855-1360, USA |
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| Abstract: | The volume, retention time, and shape of the lysozyme peak eluted from a hydrophobic interaction chromatography column (TosoHaas 650 M Phenyl) was influenced by the presence and concentration of phenylalanine in the elution buffer. Lysozyme peak retention time decreased by a factor of 2.5 with the addition of 86 mM phenylalanine to the elution buffer. |
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