Faster rates of DNA unwinding under alkaline conditions in xrs-5 cells may reflect chromatin structure alterations. |
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| Authors: | J L Schwartz E Moan R Mustafi L Fink L S Yasui |
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| Institution: | Biological and Medical Research Division, Argonne National Laboratory, IL 60439. |
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| Abstract: | The Chinese hamster ovary (CHO) cell line xrs-5 is a radiation-sensitive mutant isolated from CHO-K1 cells. The radiation sensitivity is associated with a defect in DNA double-strand break rejoining. The DNA alkaline unwinding technique was used to measure the DNA single-strand breakage caused by gamma-rays in xrs-5 and CHO-K1 cells. Greater rates of DNA unwinding were found in xrs-5 cells as compared to CHO-K1. Independent measurement of DNA strand breakage by DNA filter elution or pulsed-field gel electrophoresis failed to show any difference between the two cell lines. The greater rate of unwinding in xrs-5 cells may reflect an alteration in chromosome structure. |
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